TY - CONF
T1 - Consumption of Different Sources of Omega-3 Fatty Acids Alters Lipogenic Gene Expression in Growing Rats
AU - Mock, Kaitlin
AU - Tou, Janet C
AU - Benedito, Vagner A
AU - Gigliotti, Joseph C.
N1 - If the address matches an existing account you will receive an email with instructions to reset your password
PY - 2013/4
Y1 - 2013/4
N2 - Currently, there is an increased intake of omega-3 (n-3) polyunsaturated fatty acids (PUFA) through diet and supplementation. The objective of this study was to determine if the n-3 PUFA source plays a role in de novo lipid metabolism by altering gene expression. Female Sprague-Dawley rats were assigned to one of six diets: corn oil (CO), flaxseed oil (FO), krill oil (KO), menhaden oil (MO), salmon oil (SO), or tuna oil (TO). Most n −3 PUFA sources had decreased expression of hepatic lipogenic genes. Stearoyl CoA Desaturase 1 expression decreased in TO (P=0.04) and MO (P=0.008) compared to CO. Fatty Acid Synthase expression decreased in TO (P=0.006), SO (P=0.001), MO (P=0.001), and FO (P=0.01) compared to CO. Sterol Regulatory Element Binding Protein 1c expression decreased in MO (P=0.007) compared to CO. Peroxisome Proliferator-Activated Receptor Alpha (PPAR α) was used as a measure of hepatic lipolysis, however, no differences were found. There were no significant changes in expression of lipogenic genes in gonadal tissue of n-3 PUFA groups when compared to CO. In the gonadal tissue, PPARγ increased in FO (P=0.04) compared to CO, and Hormone Sensitive Lipase had no differences in expression compared to CO. These values suggest that n-3 fatty acids may be altering de novo lipid metabolism by decreasing expression of lipogenic genes in liver tissue, and increasing expression of a lipolytic gene in gonadal tissue.
AB - Currently, there is an increased intake of omega-3 (n-3) polyunsaturated fatty acids (PUFA) through diet and supplementation. The objective of this study was to determine if the n-3 PUFA source plays a role in de novo lipid metabolism by altering gene expression. Female Sprague-Dawley rats were assigned to one of six diets: corn oil (CO), flaxseed oil (FO), krill oil (KO), menhaden oil (MO), salmon oil (SO), or tuna oil (TO). Most n −3 PUFA sources had decreased expression of hepatic lipogenic genes. Stearoyl CoA Desaturase 1 expression decreased in TO (P=0.04) and MO (P=0.008) compared to CO. Fatty Acid Synthase expression decreased in TO (P=0.006), SO (P=0.001), MO (P=0.001), and FO (P=0.01) compared to CO. Sterol Regulatory Element Binding Protein 1c expression decreased in MO (P=0.007) compared to CO. Peroxisome Proliferator-Activated Receptor Alpha (PPAR α) was used as a measure of hepatic lipolysis, however, no differences were found. There were no significant changes in expression of lipogenic genes in gonadal tissue of n-3 PUFA groups when compared to CO. In the gonadal tissue, PPARγ increased in FO (P=0.04) compared to CO, and Hormone Sensitive Lipase had no differences in expression compared to CO. These values suggest that n-3 fatty acids may be altering de novo lipid metabolism by decreasing expression of lipogenic genes in liver tissue, and increasing expression of a lipolytic gene in gonadal tissue.
UR - https://www.fasebj.org/doi/abs/10.1096/fasebj.27.1_supplement.345.5
M3 - Presentation
T2 - FASEB Annual Meeting
Y2 - 1 April 2013
ER -