Evaluation of a Most Probable Number Quantitation System with a Modified AATCC Test Method 100 for Measuring Fungal Growth

Research output: Contribution to journalArticlepeer-review

Abstract

Industrial testing for bacterial growth on commercial substrates is highly efficient, with high-throughput means for quantifying growth. However, current antifungal testing is mostly subjective, with quantitative tests reliant on labor-intensive colony counting. We evaluated the use of a commercially available most probable number (MPN) system for quantifying growth of mold in a modified version of the AATCC Test Method (TM) 100. Using the MPN system, we were able to detect up to a 3.7-fold log reduction in mold growth between antifungal treated and untreated fabric samples. Using agar plate counts in parallel, we reproduced the trends observed using the MPN system. The trends in log reduction of growth correlated well and confirm that the MPN system satisfactorily substitutes for plate counts in evaluating antimicrobial technologies.

Original languageEnglish
Pages (from-to)11-15
Number of pages5
JournalAATCC Journal of Research
Volume2
Issue number5
DOIs
StatePublished - Sep 1 2015
Externally publishedYes

ASJC Scopus Subject Areas

  • Process Chemistry and Technology
  • Polymers and Plastics
  • Materials Chemistry

Keywords

  • AATCC test method 100
  • Antifungal
  • Antimicrobial
  • Fungi
  • Quantification

Disciplines

  • Medicine and Health Sciences

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